BLAST for Nothobranchius

This page provides BLAST search access to all the NF data produced by FLI.
query sequence:
mask nt sequence for
repeat regions:
BLAST method:
database:
expect threshold:


Descriptions

View original BLAST documentation at NCBI.


Query Sequence

Enter your sequence as plain text or in fastA format.

Expect Threshold

The statistical significance threshold for reporting matches against database sequences. The expect value given for each HSP in the BLAST report specifies the expectancy value for the number of database entries to be found merely by chance, according to the stochastic model of Karlin and Altschul (1990). If the statistical significance ascribed to a match is higher than the expect threshold, the match will not be reported. Lower expect thresholds are more stringent, leading to fewer chance matches being reported. Fractional values are acceptable.
Though you may extend the number of matching database entries by rising the expect threshold the maximum total number of entries that's reported is restricted to 120 (the best matching entries are displayed). Beside that, the threshold value is restricted to a maximum of 5.0e-4. A higher value won't add any meaningful hit to your search result (believe it).


Literature

Altschul, Stephen F., Warren Gish, Webb Miller, Eugene W. Myers, and David J. Lipman. Basic local alignment search tool. J. Mol. Biol. 215, 403-410 (1990).

Karlin, Samuel and Stephen F. Altschul (1990). Methods for assessing the statistical significance of molecular sequence features by using general scoring schemes. Proc. Natl. Acad. Sci. USA 87:2264-68.